Fig. 3

Rab10-interacting isoform of Myosin Vb regulates cell morphology during development and is essential for the apical domain expansion during compensatory cell growth. (A,C,E) Confocal scans and orthogonal (Y-Z) sections of the peridermal cells at 48 hpf of immunostained embryos of Tg(cldnB:lynEGFP) line injected with control (Control MO) and Exon D morphants (Exon D MO) (A), upon knockdown of ΔNp63 (ΔNp63 MO) and inhibition of EGF signaling by treatment with PD168393 (C) or uninjected or Exon-D morphants injected with ΔNp63 morpholino or treated with PD168393 (E). (B,D,F) Graphical representation of quantification of total, apical and basolateral surface area, and the cell height, under given genetic conditions or treatments in A,C,E, respectively. Data are median±interquartile range. **P<0.01, ***P<0.001 [Mann–Whitney U test (B); Kruskal–Wallis test with Dunn's post hoc test (D,F)]. ns, not significant. Note that only essential paired comparisons are shown in D and F using horizontal line segments. For the details of all the comparisons please refer to Table S2. Scale bars: 20 µm (X-Z plane of A,C,E, top panels); 5 µm (Y-Z plane of A,C,E, bottom panels).