Fig. 1

Myosin Vb-Rab11 interaction is essential to maintain cell size and shape, whereas Myosin Vb-Rab10 interaction controls trans-Golgi morphology. (A) Representative brightfield images of Myosin Vb morphants at 40 hpf, with and without compensation with mRNA encoding different Myosin Vb isoforms mentioned in the figure. (B) Graphical representation of proportion of Myosin Vb morphants (MyoVb MO) showing either strong, mild or no cell rounding phenotype upon injections of mRNA for the mentioned Myosin Vb isoforms. (C) Confocal images of the 40 hpf morphant peridermal cells in Tg(cldnB:lynEGFP) transgenic line with the membrane and the vesicles marked with Lyn-GFP and trans-Golgi marked with WGA (top panel confocal z-stack at 0 µm; middle panel z-stack at 1.9-2.2 µm). (D) Percentage of the morphant embryos showing trans-Golgi phenotype characterized by branching of trans-Golgi and the presence of large vesicles in the morphant embryos injected with mRNA for stated Myosin Vb isoforms. Black arrows in A show bunches of rounded-up cells. White arrow and arrowhead in C indicate a trans-Golgi vesicle and branch, respectively. Asterisks in C point to the absence of microridges in the apical domain of the peridermal cells. Horizontal bar shows mean. Scale bars: 20 µm (C).