FIGURE

Fig. 9

ID

ZDB-FIG-231212-9

Publication

Carrington et al., 2023 - Fluorescent PCR-based Screening Methods for Precise Knock-in of Small DNA Fragments and Point Mutations in Zebrafish

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Fig. 9

Examples of fluorescent PCR plots from pooled embryo screening to identify germline transmitting founders. Each embryo has a WT allele from the WT parent and an unknown allele (could be WT, indel, or knock-in) from the founder fish. Thus, a robust WT peak (denoted by green arrow) is detected in all pools with up to three additional peaks of unknown sizes. (A) Example of a positive pool with one embryo heterozygous for the knock-in allele (denoted by red arrow). Other peaks in this sample (denoted by the blue arrows) are random indels generated by NHEJ. (B) Example of a negative sample where all embryos are heterozygous for random indels (denoted by blue arrows). The x-axis shows the size of the peaks (bp), and the y-axis shows fluorescent intensity.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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