FIGURE

Fig. 3

ID

ZDB-FIG-231212-3

Publication

Carrington et al., 2023 - Fluorescent PCR-based Screening Methods for Precise Knock-in of Small DNA Fragments and Point Mutations in Zebrafish

Other Figures

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Fig. 3

Examples of CRISPR-STAT plots showing sgRNA activity. In this example, both sgRNAs selected in Figure 1 are active. sgRNA-T1 would be selected as the best target and used in subsequent steps, as it generates the DSB closer to the intended nucleotide change. The x-axis shows the size of the peaks (bp), the y-axis shows fluorescent intensity, and the green arrow denotes the WT allele.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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