FIGURE

Fig. 6

ID

ZDB-FIG-231212-6

Publication

Carrington et al., 2023 - Fluorescent PCR-based Screening Methods for Precise Knock-in of Small DNA Fragments and Point Mutations in Zebrafish

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Fig. 6

Schematic showing design of screening primers for insertion of a DNA fragment. Primers should not overlap with the HDR template to allow for screening of precise integration. Homology arms (left HA and right HA) are marked in red in both genomic DNA and HDR template. Inserted sequence is marked in blue on the HDR template. M13F sequence is added to the forward primer and the PIG-tail sequence is added to the reverse primer.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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