FIGURE

Fig. 7

ID

ZDB-FIG-231212-7

Publication

Carrington et al., 2023 - Fluorescent PCR-based Screening Methods for Precise Knock-in of Small DNA Fragments and Point Mutations in Zebrafish

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Fig. 7

Schematic showing design of screening primers for knock-in of a point mutation. Primers should not overlap with the HDR template (shown with homology arms in red, restriction enzyme recognition sequence in blue, and point mutation by black line) to allow for screening of precise integration. M13F sequence is added to the 5′ end of forward primer. PIG-tail and the restriction enzyme recognition sequences (RS) are added to the 5′ end of reverse primer.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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