FIGURE

Fig. 6.

ID

ZDB-FIG-231118-23

Publication

Li et al., 2023 - The RNA-binding protein CSDE1 promotes hematopoietic stem and progenitor cell generation via translational control of Wnt signaling

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Fig. 6.

Csde1 regulates Wnt/β-catenin signaling activity to control HSPC generation. (A) Expression of runx1 and cmyb (arrowheads) in the AGM region in WT siblings, csde1 mutants and csde1 mutants injected with fli1a:vp16-tcf7l1ΔN-tdTomato constructs at 36 hpf by WISH (left panels) with quantification (right panel). (B) qPCR showing the expression of runx1, cmyb and Wnt signaling genes at 36 hpf in control embryos, csde1 morphants and csde1 morphants injected with fli1a:vp16-tcf7l1ΔN-tdTomato constructs at 36 hpf. (C) Confocal imaging (left panels) showing that endothelial-derived-tcf7l1ΔN-tdTomato overexpression rescued the population of cmyb⁺ HSPCs (white arrowheads), compared with csde1 morphants at 36 hpf, with quantification (right panel). Data are mean±s.d. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 (two-tailed unpaired Student's t-test). n≥3 replicates. Numbers indicate the number of embryos with respective phenotype/total number of embryos analyzed in each experiment (A,C). Scale bars: 100 μm (A); 50 μm (C).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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