FIGURE
Fig. 1
- ID
- ZDB-FIG-231110-32
- Publication
- Brown et al., 2023 - Genetically Encoded Aminocoumarin Lysine for Optical Control of Protein-Nucleotide Interactions in Zebrafish Embryos
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Fig. 1
Incorporation of ACK and AC2K into proteins in mammalian cells. (A) Chemical structures of HCK, HC2K, ACK, and AC2K. The photolabile group/fluorophore is colored blue. (B) Confirmation of ACK and AC2K incorporation into a reporter construct through fluorescence imaging in HEK293T cells (10× magnification) and western blot. (C) Expression of NLS-EGFP-AC2K in live NIH 3T3 cells (20× magnification). (D) Fixed HeLa cells expressing NLS-EGFP-ACK (top row, 40×) and NLS-EGFP-AC2K (bottom row, 63×). Cells are counterstained with rhodamine–phalloidin. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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