ZFIN Image: Brown et al., 2023, Fig. 1

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Figure Caption

Fig. 1 Incorporation of ACK and AC₂K into proteins in mammalian cells. (A) Chemical structures of HCK, HC₂K, ACK, and AC₂K. The photolabile group/fluorophore is colored blue. (B) Confirmation of ACK and AC₂K incorporation into a reporter construct through fluorescence imaging in HEK293T cells (10× magnification) and western blot. (C) Expression of NLS-EGFP-AC₂K in live NIH 3T3 cells (20× magnification). (D) Fixed HeLa cells expressing NLS-EGFP-ACK (top row, 40×) and NLS-EGFP-AC2K (bottom row, 63×). Cells are counterstained with rhodamine–phalloidin.

Acknowledgments

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