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Fig. S6

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ZDB-FIG-231020-35

Publication

Regot et al., 2014 - High-sensitivity measurements of multiple kinase activities in live single cells

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Fig. S6

JNK KTR Enables the Measurement of Dynamic JNK Kinase Activity within the Innate Immune Signaling Network, Related to Figure 5

(A) Clonal line 3B8 (RelA^?/? KO 3T3, H2B-EGFP, p65-DsRed and JNK KTR-mCerulean3) was stimulated with indicated concentrations of TNF?, IL-1? or LPS, imaged and quantified as described in Methods. Heat maps represent clustered individual cells with normalized JNK KTR and nuclear p65 (n-p65) dynamics displayed in tandem. Each condition represents a minimum of 300 cells obtained from 3 independent experiments.

(B and C) Average population dynamics from data obtained for Panel a. Data represent the mean � SD from more than 300 cells per condition obtained from 3 independent experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 157, Regot, S., Hughey, J.J., Bajar, B.T., Carrasco, S., Covert, M.W., High-sensitivity measurements of multiple kinase activities in live single cells, 172417341724-34, Copyright (2014) with permission from Elsevier. Full text @ Cell