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Fig. S1

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ZDB-FIG-231018-41

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Regot et al., 2014 - High-sensitivity measurements of multiple kinase activities in live single cells

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Fig. S1

c-Jun Fragment Analysis, Related to Figure 1

(A) 3T3 cells (wild-type or expressing indicated c-Jun fragments fused to Clover) were stimulated with anisomycin (50 ng/ml) and harvested at indicated time points for Western Blot analysis. Representative of 2 independent experiments.

(B) Cell lines used in Panel a were stimulated with anisomycin (50 ng/ml), imaged and quantified as described in Methods. Schematic representation of each fragment is shown for clarity. Data represent the mean � SD from the indicated number of cells obtained from 2 independent experiments.

(C) 3T3 cells (wild-type or expressing indicated c-Jun^29?84 mutants) were stimulated with anisomycin (50 ng/ml) and harvested at indicated time points for Western Blot analysis. Representative of 2 independent experiments.

(D) Cell lines used in Panel a were stimulated with anisomycin (50 ng/ml), imaged and quantified as described in Methods. Data represent the mean � SD from more than 100 cells for each mutant obtained from 2 independent experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 157, Regot, S., Hughey, J.J., Bajar, B.T., Carrasco, S., Covert, M.W., High-sensitivity measurements of multiple kinase activities in live single cells, 172417341724-34, Copyright (2014) with permission from Elsevier. Full text @ Cell