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The morphology of gut endoderm in 48 hpf embryos. (A-B) Images of a live embryo in which the endoderm is labeled with EGFP. (A) Overlay image of brightfield and epifluorescence of EGFP, Lateral view. (B) Epifluorescence EGFP image. Dorsal view. (C-G′) Confocal images taken from a flat-mounted sample at a similar region outline in B (highlighted by yellow rectangle), showing the expression of actin (detected by Phalloidin staining) in memGFP-labelled gut endoderm. Yellow arrowheads, actin-enriched gut lumen. (C-D′) Images of XY view. (C) Z projection image. (D-D′) Single plane images. (E-G′) Images of XZ planes taken at the positions marked by i-iii in D. White arrowheads, actin enriched sites. A, anterior; P, posterior; V, ventral; D, dorsal; ML; mediolateral.
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