FIGURE

Fig. 3

ID

ZDB-FIG-230828-94

Publication

Domingues et al., 2023 - Cholesteryl hemiazelate Identified in Cardiovascular Disease Patients Causes in vitro and in vivo Inflammation

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Fig. 3

ChA affects the cytokine profile of neutrophils. Neutrophils were harvested from human volunteers (five different donors) using a gradient with histopaque-1177 and 1009. Cells were cultured in the presence of ChA (25, 100, and 500 μM) or vehicle for 4 h. After treatment, neutrophils were stained with monoclonal antibodies to determine the expression of CD11b (A) and cytokines: IL-1β (B), TNF-α (C), IL-6 (D), and IL-10 (E); data represent the percentage of positive cells for each inflammatory marker normalized to the control cells. ∗P < 0.05; ∗∗P < 0.01 using one-way ANOVA with Dunnett’s multiple comparisons test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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