FLVCR1a expression is enhanced in angiogenic endothelial cells (aECs). A qRT-PCR analysis showing Flvcr1a mRNA levels in wild-type mouse embryos at different developmental stages (i.e., embryonic day (E) 9.5, 11.5, E13.5, E15.5). n = 4. B qRT-PCR analysis showing Flvcr1a transcript levels in embryonic ECs (CD31+) and non-endothelial fractions (CD31−) collected from mouse embryos at E9.5 and E13.5. n = 3. C qRT-PCR analysis showing Flvcr1a mRNA levels in embryonic angiogenic ECs (E9.5) and tumor-associated ECs (TECs) from Lewis Lung Carcinoma Cells (LLC)-xenografts. D qRT-PCR analysis showing Flvcr1a transcript levels in mouse ECs isolated from adult lung and LLC tumors. E, F Representative Western Blot analysis (E) and quantification (F) of FLVCR1a-myc-tagged protein expression in murine ECs from adult lung and LLC tumor. GFLVCR1a expression levels in human qECs (capillary ECs) and aECs (immature/stalk and tip cells) isolated from lung adenocarcinoma. Data were taken from public available database. aECs angiogenic ECs, NEC/TEC Normal/Tumor ECs, RQ Relative Quantification. Data are representative of at least 3 independent experiments and are expressed as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For statistical analyses, ordinary one-way ANOVA test with Tukey’s multiple comparisons (A, B), parametric unpaired t test (C, D, F), and Mann–Whitney test (G) were used
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