FIGURE

Fig. 2

ID

ZDB-FIG-230710-36

Publication

Petrillo et al., 2023 - Endothelial cells require functional FLVCR1a during developmental and adult angiogenesis

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Fig. 2

Flvcr1a targeting impairs developmental angiogenesis in mouse and zebrafish embryos. A(i) Phenotype of E13.5 control (Flvcr1a^fl/fl) and Flvcr1a endothelial-specific constitutive knockout (Flvcr1a^fl/fl;Tie2-Cre) mouse embryos. (ii) Whole-mount staining with CD31/Pecam-1 antibody showing the embryonic vasculature in E11.5 embryos. Enlarged pictures of forelimbs are shown. White dashed line refers to the transverse section displayed in panels iii-iv. (iii-iv) Transverse sections of E11.5 forelimb from Flvcr1a^fl/fl and Flvcr1a^fl/fl;Tie2-Cre embryos stained with CD31/Pecam-1 antibody. B Quantification of the average vessel size in Flvcr1a^fl/fl and Flvcr1a^fl/fl;Tie2-Cre limbs transverse sections shown in (iii). C, D Photographs of transgenic (Tg) kdrl:GFP zebrafish embryos injected with CTRL, MO-ATG, or MOl1EX2 morpholinos alone or with Flvcr1a cRNA. Images were collected at 48 hpf. Magnification of intersegmental vessels in controls, morphants and rescued embryos, are shown. ****p < 0.0001. For statistical analyses, parametric unpaired t test was used. A.U. arbitrary units, MO morpholino, hpf hours post fertilization

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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