Fig. 4

Endothelial Flvcr1a is dispensable in adult healthy tissues. A Representative histological sections of lung and liver tissue from Flvcr1a^fl/fl and Flvcr1a^fl/fl;Cdh5-Cre^ERT2 mice. (i, ii) Tissue sections stained with hematoxylin and eosin (HE) to look at the cellular morphology. Scale bar: 200 µm. (iii) Confocal images of liver sections from Flvcr1a^fl/fl and Flvcr1a^fl/fl;Cdh5-Cre^ERT2 stained with CD31 antibody (green) and DAPI (blu) to look at vascular network organization. Scale bar: 200 µm. B Liver vascular area quantification expressed as % of liver area positive to CD31. C–F Gadolinium-based DCE-MRI analysis to evaluate (C) the total vascular volume (Vp) and D–F vessel permeability in Flvcr1a^fl/fl and Flvcr1a^fl/fl;Cdh5-Cre^ERT2 livers. HE, hematoxylin/eosin DCE-MRI, Dynamic Contrast-Enhanced Magnetic Resonance Imaging. Data are representative of at least 3 independent experiments and are expressed as mean ± SEM. **p < 0.01; ***p < 0.001; ns not significant. For statistical analyses, an unpaired Student’s t test was used