FIGURE

Fig. 4

ID

ZDB-FIG-230616-20

Publication

Yan et al., 2022 - Fluorescence intensity and lifetime imaging of lipofuscin-like autofluorescence for label-free predicting clinical drug response in cancer

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Lipofuscin autofluorescence enables label-free detecting treatment responses in 3D spheroids and organoids. A Two-photon fluorescence image of lipofuscin red autofluorescence in 3D spheroids before and after treatment with cisplatin (5, 15, and 30 μM) for 2 days. The insets show the 3D view in spheroids. B Average TPF intensities of cells in the control group and treated group. Data are the mean ± SD of three independent experiments. C Lifetime traces of lipofuscin red fluorescence in the 30 μM cisplatin-treated and control cells. D Two-photon fluorescence images of lipofuscin red autofluorescence in organoids before and after treatment with cisplatin (1, 5, and 30 μM) for 4 days. The insets show the 3D view in organoids. E Average TPF intensities of cells (n = 30) in the control group and treated group. Data are the mean ± SD of three independent experiments. F Lifetime traces of lipofuscin red fluorescence of the 30 μM cisplatin-treated and control groups after 4 days treatment.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Redox Biol.