Fig. 2

Fig. 2. Elevated exosomal linc-ROR promotes angiogenesis of HUVECs. (A) CNE2 cells were transfected with shROR1, shROR2, shROR3 and a negative control. After 48 h, total RNA from CNE2 cells was isolated and qRT?PCR for linc-ROR was performed. (B?C) qRT-PCR and fluorescence microscope image (Scale bar: 100 ?m) exhibited the transfection efficiency of shROR lentivirus and a negative control lentivirus in CNE2. (D?I) Cell proliferation and migration were detected in HUVECs treated with exosomes from CNE2-NC and CNE2-shROR culture medium. The CCK8 (D) and EDU assay (scale bar: 50 ?m) (E, F) were carried out to measure cell proliferation while transwell assays (scale bar: 100 ?m) (G, H) and wound healing assay (scale bar: 100 ?m) (I) were carried out to measure cell mobility. (J?K) Tube formation assay was used to detect the angiogenesis ability of HUVECs co-cultured with exosomes from CNE2-NC and CNE2-shROR culture medium (scale bar: 100 ?m). All data show mean ± SD of at least three independent experiments. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).