PUBLICATION
Tumor-derived exosomal lincRNA ROR promotes angiogenesis in nasopharyngeal carcinoma
- Authors
- Zhang, S., Cai, J., Ji, Y., Zhou, S., Miao, M., Zhu, R., Li, K., Xue, Z., Hu, S.
- ID
- ZDB-PUB-221004-4
- Date
- 2022
- Source
- Molecular and Cellular Probes 66: 101868 (Journal)
- Registered Authors
- Keywords
- Angiogenesis, Exosome, Linc-ROR, LincRNA, Nasopharyngeal carcinoma
- MeSH Terms
-
- Cell Proliferation/genetics
- Animals
- Nasopharyngeal Neoplasms*/genetics
- Zebrafish/genetics
- Zebrafish/metabolism
- RNA, Long Noncoding*/genetics
- RNA, Long Noncoding*/metabolism
- Nasopharyngeal Carcinoma/genetics
- Cell Line, Tumor
- Exosomes*/genetics
- Exosomes*/metabolism
- PubMed
- 36183926 Full text @ Mol. Cell. Probes
Citation
Zhang, S., Cai, J., Ji, Y., Zhou, S., Miao, M., Zhu, R., Li, K., Xue, Z., Hu, S. (2022) Tumor-derived exosomal lincRNA ROR promotes angiogenesis in nasopharyngeal carcinoma. Molecular and Cellular Probes. 66:101868.
Abstract
Long intergenic noncoding RNAs (lincRNAs) are expressed aberrantly in several malignancies, including nasopharyngeal carcinoma (NPC), where linc-ROR expression was found to be elevated. Being a hallmark of malignant tumors, angiogenesis has prompted us to investigate the impact of linc-ROR on NPC angiogenesis. This study demonstrates that linc-ROR is substantially expressed in serum exosomes from NPC and can be taken up by HUVECs. Using qRT-PCR, the CCK8 test, the transwell migration assay, the wound healing assay, and the tube formation assay, we demonstrated that linc-ROR increases proliferation, migration, and angiogenesis in vitro. Similar to prior research, our results have shown that linc-ROR can stimulate tumor angiogenesis in the zebrafish model. Thus, the p-AKT/p-VEGFR2 pathway is the mechanism by which linc-ROR affects the aforementioned biological activities. By stimulating angiogenesis, linc-ROR appears to play a significant role in the course of NPC and could account for a therapeutic target.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping