FIGURE 2

Proliferative cells of regenerating retinas include careg:EGFP-positive cells with an elongated nuclei. Sections of careg:EGFP (green) retinas, immunostained for the G1/S-phase cell cycle marker PCNA (red) and the nuclear marker DAPI (blue). Expression of careg:EGFP (green) is absent in the uninjured retina, but it is induced at 2 dpMNU and persists until 30 dpMNU. In the inner nuclear layer (INL), EGFP/PCNA double positive cells with an elongated nucleus (arrows) can be observed at 2, 3, and 7 dpMNU. Single EGFP-negative and PCNA-positive cells with roundish nuclei (arrowheads) are observed at all time-points after injury. Clusters of EGFP-negative and PCNA-positive cells (encircled with a dashed line) correspond to progenitor cells at 3 and 7 dpMNU. At 15 and 30 dpMNU, PCNA-positive cells are present in the outer nuclear layer (ONL). At 90 dpMNU, no EGFP-positive cells are detected. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; rONL, rod outer nuclear layer; cONL, cone outer nuclear layer; PRL, photoreceptors layer. N ≥ 3 (number of fish).