Figure 6
- ID
- ZDB-FIG-221226-285
- Publication
- Ruzic et al., 2022 - Discovery of 1-Benzhydryl-Piperazine-Based HDAC Inhibitors with Anti-Breast Cancer Activity: Synthesis, Molecular Modeling, In Vitro and In Vivo Biological Evaluation
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Inhibition of cell survival and growth of MDA-MB-231 tumor spheres treated with synthesized compounds. After 4 d, formed spheres were treated with synthesized compounds and tubastatin A at equimolar concentration (50 μM) for 72 h. The formation and growth of tumor spheres were examined and imaged with an Olympus CKX53, using 4×/0.13 and 10×/0.25 objectives. Scale bar: 100 μm. (A) MDA-MB-231 tumor spheres observed under the bright field, and (B) the cytotoxicity of compounds toward the MDA-MB-231 tumor spheres was investigated by MTT assay. (C) MDA-MB-231 cells were co-cultured with the tested compounds (IC50 and 50 µM) in sphere culture conditions for 4 d. The formation and growth of tumor spheres were examined and imaged with an Olympus CKX53, using a 4×/0.4 objective. Scale bar: 200 μm. (D) The cytotoxicity of compounds in the co-treatment with MDA-MB-231 tumor spheres was investigated by MTT assay. |