|
Figure 6 Inhibition of cell survival and growth of MDA-MB-231 tumor spheres treated with synthesized compounds. After 4 d, formed spheres were treated with synthesized compounds and tubastatin A at equimolar concentration (50 μM) for 72 h. The formation and growth of tumor spheres were examined and imaged with an Olympus CKX53, using 4×/0.13 and 10×/0.25 objectives. Scale bar: 100 μm. (A) MDA-MB-231 tumor spheres observed under the bright field, and (B) the cytotoxicity of compounds toward the MDA-MB-231 tumor spheres was investigated by MTT assay. (C) MDA-MB-231 cells were co-cultured with the tested compounds (IC50 and 50 µM) in sphere culture conditions for 4 d. The formation and growth of tumor spheres were examined and imaged with an Olympus CKX53, using a 4×/0.4 objective. Scale bar: 200 μm. (D) The cytotoxicity of compounds in the co-treatment with MDA-MB-231 tumor spheres was investigated by MTT assay.