FIGURE

Fig 4

ID

ZDB-FIG-221216-71

Publication

Liu et al., 2022 - LOF variants identifying candidate genes of laterality defects patients with congenital heart disease

Other Figures

All Figure Page

Back to All Figure Page

Fig 4

The expression patterns of spaw, lefty2, and pitx2 at 18–24 hpf.

(A, C) the expression of spaw and pitx2 exhibit four patterns in LPM: left (normal), right (abnormal), bilateral (abnormal), or absent (abnormal). (B) The expression of lefty2 shows four patterns in the cardiac field: left (normal), right (abnormal), bilateral (abnormal), or absent (abnormal). (D-F) Summary of spaw, lefty2, and pitx2 mRNA expression in zebrafish morphants. Embryos are viewed dorsally with anterior to the top. Each experiment was repeated at least 3 times with > 50 embryos examined for each group every time. Chi-squared test (continuity corrected) was used in D, E and F; *P <0.05, **P < 0.01, ***P < 0.001, respectively vs. Std-16ng (glant11, trip11, dnhd1, and cfap74) or Std-2ng (egr4).

Expression Data

Genes:	lft2 pitx2 spaw
Fish:	AB + MO1-cacna1db AB + MO1-dnhd1 AB + MO1-egr4 AB + MO1-trip11
Knockdown Reagents:	MO1-cacna1db MO1-dnhd1 MO1-egr4 MO1-trip11
Anatomical Term:	lateral plate mesoderm
Stage Range:	14-19 somites to 26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-cacna1db AB + MO1-dnhd1 AB + MO1-trip11
Knockdown Reagents:	MO1-cacna1db MO1-dnhd1 MO1-trip11
Observed In:	lateral plate mesoderm
Stage Range:	14-19 somites to 26+ somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.