FIGURE

Fig 5

ID

ZDB-FIG-221216-72

Publication

Liu et al., 2022 - LOF variants identifying candidate genes of laterality defects patients with congenital heart disease

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Fig 5

Candidate genes are required for KV formation and ciliogenesis.

(A) The light-micro graph at the 8-somite stage showed different KV sizes, including normal and small. (B) The vesicle size of KV apical area. N?19. (C) Fluorescent immunostaining of cilia in KV using anti-?-Tubulin antibodies at the 12hpf. (D) LRO Cilia number per KV was quantified and the group values were expressed as the mean ± SD. N?15. (E) The average length of LRO Cilia per KV was quantified and the group values were expressed as the mean ± SD. N?7. (F) The ciliary beat frequency (CBF). N?12. Statistical significance was determined by Mann Whitney test; *P <0.05, ***P < 0.001, respectively vs. Std.

Expression Data

Antibody:	Ab1-tuba
Fish:	AB + MO1-cacna1db AB + MO1-dnhd1 AB + MO1-trip11
Knockdown Reagents:	MO1-cacna1db MO1-dnhd1 MO1-trip11
Anatomical Term:	Kupffer's vesicle cilium
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-cacna1db AB + MO1-dnhd1 AB + MO1-trip11
Knockdown Reagents:	MO1-cacna1db MO1-dnhd1 MO1-trip11
Observed In:	Kupffer's vesicle Kupffer's vesicle cilium
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

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