Fig 4
(A, C) the expression of spaw and pitx2 exhibit four patterns in LPM: left (normal), right (abnormal), bilateral (abnormal), or absent (abnormal). (B) The expression of lefty2 shows four patterns in the cardiac field: left (normal), right (abnormal), bilateral (abnormal), or absent (abnormal). (D-F) Summary of spaw, lefty2, and pitx2 mRNA expression in zebrafish morphants. Embryos are viewed dorsally with anterior to the top. Each experiment was repeated at least 3 times with > 50 embryos examined for each group every time. Chi-squared test (continuity corrected) was used in D, E and F; *P <0.05, **P < 0.01, ***P < 0.001, respectively vs. Std-16ng (glant11, trip11, dnhd1, and cfap74) or Std-2ng (egr4).