FIGURE

Fig 6

ID

ZDB-FIG-221121-13

Publication

Sarmah et al., 2022 - Elf3 deficiency during zebrafish development alters extracellular matrix organization and disrupts tissue morphogenesis

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Fig 6

Dysregulation of extracellular matrix proteins in Elf3 morphants.

(A) IPA predicted direct relationships and downstream targets of Elf3. (B-D) In situ hybridization detecting sox9b expression at 2 dpf show strong expression in the retina, brain ventricular zone, craniofacial cartilages, and pectoral fin in the control embryos (B) and weaker expression in those areas in the morphants (C, D). arrow: developing craniofacial cartilages. (E) Quantitative RT-PCR assays comparing transcripts of mmp13, mmp9, mmp2, fn, and col2α1 (average fold change from at least 3 independent experiments) show altered levels of expression of those genes in morphants compared to control, Student’s t-test: P<0.05. Scale bar for B-D = 200 μm.

Expression Data

Genes:	col2a1a fn1a mmp2 mmp9 mmp13a sox9b
Fish:	AB AB + MO1-elf3 AB + MO2-elf3
Knockdown Reagents:	MO1-elf3 MO2-elf3
Anatomical Terms:	cranial cartilage pectoral fin retina ventricular zone whole organism
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-elf3 AB + MO2-elf3
Knockdown Reagents:	MO1-elf3 MO2-elf3
Observed In:	cranial cartilage pectoral fin retina ventricular zone whole organism
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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