FIGURE

Fig. 7

ID
ZDB-FIG-221119-33
Publication
Coffey et al., 2021 - Lysosomal Function Impacts the Skeletal Muscle Extracellular Matrix
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Fig. 7

mmp9 mRNA and protein are upregulated in spns1?/? larvae at 3.5 dpf. (A,B) Anterior left, dorsal top, side-mounted larvae. Phalloidin (white) to visualize actin and MMP-9 antibody staining (pseudo-colored fuchsia). (A,A2,B,B2) Merged phalloidin and MMP-9 channels and (A1,B1,A3,B3) MMP-9 single channel. (A1) MMP-9 is not detected in WT muscle. (B1) MMP-9 is detected in spns1?/? muscle. White arrowheads highlight MMP-9 staining (or lack thereof in wild-type larvae). (C) Relative mmp9 mRNA expression in WT and spns1?/? larvae measured as fold change in 3.5 dpf larvae. (D) Percentage of spns1?/? and WT at 1 and 3.5 dpf with qualitatively scored relative fluorescence intensity. ** p < 0.01, **** p < 0.0001. Scale bars 50 µm.
Expression Data
Gene:
Fish:
Anatomical Terms:
Stage: Protruding-mouth

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Protruding-mouth

Phenotype Detail
Acknowledgments
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