FIGURE

Fig. 5

ID

ZDB-FIG-221119-31

Publication

Coffey et al., 2021 - Lysosomal Function Impacts the Skeletal Muscle Extracellular Matrix

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

The laminin receptor integrin α7 is not required for laminin-111 protein re-expression at 3.5 dpf in spns1^−/− larvae but does contribute to muscle resilience in spns1^−/− larvae. (A–D) Anterior left, dorsal top, side-mounted larvae. Phalloidin (white) and laminin-111 (cyan) stained WT, itga7^−/−, spns1^−/−, and spns1^−/−; itga7^−/− larvae at 3.5 dpf. (A–D) Merged phalloidin and laminin-111 channels and (A1–D1) single laminin-111 channel. White arrows indicate the MTJ. Red arrowheads point to dystrophic lesions. Yellow star indicates aberrant laminin-111 surrounding detached fibers. (E) Percent of myotomes with dystrophic lesions. Note the significant increase in muscle degeneration in spns1^−/−; itga7^−/− larvae compared to itga7^−/− single mutants. * p < 0.05, **** p < 0.0001. Scale bar 50 µm.

Expression Data

Antibody:	Ab1-lam
Fish:	WT itga7^mai18/mai18 spns1^{hi891Tg/hi891Tg} itga7^mai18/mai18; spns1^{hi891Tg/hi891Tg}
Anatomical Term:	vertical myoseptum muscle tendon junction
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	itga7^mai18/mai18 spns1^{hi891Tg/hi891Tg} itga7^mai18/mai18; spns1^{hi891Tg/hi891Tg}
Observed In:	myotome skeletal muscle cell vertical myoseptum muscle tendon junction
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J Dev Biol