FIGURE

Fig. 2

ID

ZDB-FIG-221119-28

Publication

Coffey et al., 2021 - Lysosomal Function Impacts the Skeletal Muscle Extracellular Matrix

Other Figures

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Fig. 2

Normal initial muscle development is followed by abnormal 3.5 dpf laminin-111 levels in spns1^−/− embryos and larvae. (A1–H3) Anterior left, dorsal top, side-mounted embryos and larvae stained with phalloidin (white) to visualize actin and laminin-111 antibodies (cyan). (A1–H1) Phalloidin staining. (A2–H2) Laminin-111 staining. (A3–H3) Merged phalloidin and laminin-111 channels. White arrowheads point to laminin-111 localized to the MTJ. Laminin-111 staining, not detected in WT embryos and larvae at 3–3.5 dpf, is detected in (H2–H3) spns1^−/− 3.5 dpf larvae. (I,J) Relative fluorescent intensity of laminin-111 protein in WT and spns1^−/− embryos and larvae over time (see methods). * p < 0.05, ** p < 0.01, **** p < 0.0001. Scale bars 50 µm.

Expression Data

Antibody:	Ab1-lam
Fish:	WT spns1^{hi891Tg/hi891Tg}
Anatomical Term:	vertical myoseptum muscle tendon junction
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	spns1^{hi891Tg/hi891Tg}
Observed In:	vertical myoseptum muscle tendon junction
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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