Fig. 5

Fig. 5

The laminin receptor integrin α7 is not required for laminin-111 protein re-expression at 3.5 dpf in spns1^−/− larvae but does contribute to muscle resilience in spns1^−/− larvae. (A–D) Anterior left, dorsal top, side-mounted larvae. Phalloidin (white) and laminin-111 (cyan) stained WT, itga7^−/−, spns1^−/−, and spns1^−/−; itga7^−/− larvae at 3.5 dpf. (A–D) Merged phalloidin and laminin-111 channels and (A1–D1) single laminin-111 channel. White arrows indicate the MTJ. Red arrowheads point to dystrophic lesions. Yellow star indicates aberrant laminin-111 surrounding detached fibers. (E) Percent of myotomes with dystrophic lesions. Note the significant increase in muscle degeneration in spns1^−/−; itga7^−/− larvae compared to itga7^−/− single mutants. * p < 0.05, **** p < 0.0001. Scale bar 50 µm.