FIGURE

Figure 7

ID

ZDB-FIG-221029-78

Publication

Haynes et al., 2022 - KLC4 shapes axon arbors during development and mediates adult behavior

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Figure 7

Midline crossing of apical protrusions occurs more frequently when KLC function is disrupted.

(A–B) Confocal projections (dorsal views) of partial z-stacks containing only the optical sections in the plane of the RB cell bodies within the spinal cord in a wild-type Tg(–3.1ngn1:gfp-caax) embryo (A), or a klc4^uw314 Tg(–3.1ngn1:gfp-caax) embryo (B), with an apical protrusion from a neuron crossing the midline and reaching the contralateral side (white arrowhead). Scale bars = 20 µm. (C) The percentage of neurons per embryo that had an apical protrusion crossing the midline of the spinal cord was quantified. **p<0.0052, Mann–Whitney test. Mean with SEM displayed over individual data points. Wild type N=608 neurons from 21 embryos, klc4^uw314 N=1496 neurons from 47 embryos, 2% DMSO N=429 neurons from 15 embryos, Kinesore N=595 neurons from 21 embryos. (D–F) Mosaic labeling of individual neurons by injection with –3.1ngn1:gfp-caax DNA into klc4^uw314 mutants (D), DMSO-treated embryos I or kinesore-treated embryos (F) showing that apical protrusions (magenta arrowheads) can fasciculate onto the contralateral central axon.

Expression Data

Gene:	GFP
Fish:	uw4Tg (AB/TL) klc4^uw314/uw314; uw4Tg (AB)
Condition:	chemical treatment by environment: 3,5-Dibromo-N'-((2,5-dimethyl-1-(3-nitrophenyl)-1H-pyrrol-3-yl)methylene)-4-hydroxybenzohydrazide
Anatomical Term:	Rohon-Beard neuron axon
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	uw4Tg (AB/TL) klc4^uw314/uw314; uw4Tg (AB)
Condition:	chemical treatment by environment: 3,5-Dibromo-N'-((2,5-dimethyl-1-(3-nitrophenyl)-1H-pyrrol-3-yl)methylene)-4-hydroxybenzohydrazide
Observed In:	Rohon-Beard neuron axon Rohon-Beard neuron axon extension involved in axon guidance Rohon-Beard neuron cell body
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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