PUBLICATION
KLC4 shapes axon arbors during development and mediates adult behavior
- Authors
- Haynes, E.M., Burnett, K.H., He, J., Jean-Pierre, M.W., Jarzyna, M., Eliceiri, K.W., Huisken, J., Halloran, M.C.
- ID
- ZDB-PUB-221018-32
- Date
- 2022
- Source
- eLIFE 11: (Journal)
- Registered Authors
- Halloran, Mary, Huisken, Jan
- Keywords
- cell biology, neuroscience, zebrafish
- MeSH Terms
-
- Animals
- Axons/physiology
- Kinesins*/genetics
- Morphogenesis
- Sensory Receptor Cells/physiology
- Zebrafish*
- PubMed
- 36222498 Full text @ Elife
Citation
Haynes, E.M., Burnett, K.H., He, J., Jean-Pierre, M.W., Jarzyna, M., Eliceiri, K.W., Huisken, J., Halloran, M.C. (2022) KLC4 shapes axon arbors during development and mediates adult behavior. eLIFE. 11:.
Abstract
Development of elaborate and polarized neuronal morphology requires precisely regulated transport of cellular cargos by motor proteins such as kinesin-1. Kinesin-1 has numerous cellular cargos which must be delivered to unique neuronal compartments. The process by which this motor selectively transports and delivers cargo to regulate neuronal morphogenesis is poorly understood, although the cargo-binding kinesin light chain (KLC) subunits contribute to specificity. Our work implicates one such subunit, KLC4, as an essential regulator of axon branching and arborization pattern of sensory neurons during development. Using live imaging approaches in klc4 mutant zebrafish, we show that KLC4 is required for stabilization of nascent axon branches, proper microtubule (MT) dynamics, and endosomal transport. Furthermore, KLC4 is required for proper tiling of peripheral axon arbors: in klc4 mutants, peripheral axons showed abnormal fasciculation, a behavior characteristic of central axons. This result suggests that KLC4 patterns axonal compartments and helps establish molecular differences between central and peripheral axons. Finally, we find that klc4 mutant larva are hypersensitive to touch and adults show anxiety-like behavior in a novel tank test, implicating klc4 as a new gene involved in stress response circuits.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping