A 3 dpf larva is touched on the tail using a gel loading tip, provoking a swim response. (B) Larval response time was measured starting from beginning of swim to cessation of motion. (C) The number of swim bouts performed by each larva during total post-touch swim time was counted. For B-C, each point represents an individual larva. ***p=0.0005, Mann-Whitney test. Error bars = SEM. Wild type N=45, klc4uw314 N=41. 55 total larva were tested for each genotype, but larva that did not respond were not included in these data. (D) Schematic of the novel tank test design, with upper and lower zones demarcated by the center dotted line. (E) Percentage of fish per experiment that entered the upper zone of the tank during a 3-min exposure to a novel tank. Error bars = SEM. Each point represents one experiment day: wild type N=3 experiments with 30 total fish, klc4uw314 N=3 experiments with 39 total fish. (F) The time it took for individual fish to first cross into the upper zone during a 3-min exposure to a novel tank. Points represent individual fish, error bars = SEM. Wild type N=24, klc4uw314 N=8. Fish that did not cross into the upper zone are not represented in this data. NS by t-test, p=0.7766. (G) The total time fish spent in the upper zone of the novel tank during a 3-min exposure. Points represent individual fish, ***p=0.0002 by t-test, error bars = SEM. Wild type N=30, klc4uw314 N=39. (H) Percentage of fish to freeze per experiment during a 3-min exposure to the novel tank. Error bars = SEM. Each point represents one experiment day: wild type N=3 experiments with 30 total fish, klc4uw314 N=3 experiments with 39 total fish. (I) The total time fish spent frozen during a 3-min exposure to a novel tank. Points represent individual fish, ****p<0.0001 by Mann-Whitney test, error bars = SEM. Wild type N=30, klc4uw314 N=39.
