(A) Diagram of the highly conserved domains of KLC4. Arrows indicate where mutation occurs in humans and in our zebrafish mutant. (B) Qualitative RT-PCR showing both long and short klc4 isoforms are expressed throughout development. (C–D) In situ hybridization for klc4 mRNA shown in lateral view (C) or dorsal view (D) of the head in wild type 24 hpf embryos. (E) Lateral view of trunk showing klc4 expression in Rohon-Beard sensory neurons in a 24 hpf wild type embryo. (F–G) klc4 expression in the lateral view (F) or dorsal view (G) of the head of a 48 hpf wild type embryo. (H) Lateral view of trunk showing klc4 expression in the spinal cord of a 48 hpf wild type embryo. (I–K) Lateral (I,K) and dorsal (J) views of klc4 long isoform specific in situ hybridization in wild type 24 hpf embryos. Lateral (L,N) and dorsal (M) views of klc4 in situ hybridization in 24 hpf klc4uw314 mutant embryo head (L,M) and trunk (N) showing little expression of klc4. Te = telencephalon, dien = diencephalon, mb = midbrain, hb = hindbrain, tg = trigeminal ganglion, pllg = posterior lateral line ganglion, rb = rohon beard neuron.
