FIGURE

Fig. 6

ID

ZDB-FIG-220926-14

Publication

Zoodsma et al., 2022 - Disruption of grin2B, an ASD-associated gene, produces social deficits in zebrafish

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Fig. 6

Nonsense-mediated decay and compensatory changes in NMDAR subunit expression in grin2B^−/−. A Relative rt-qPCR expression levels at grin2Ba at 3 dpf (n = 4, 4) and 5 dpf (n = 4, 4; ***p = 3.5e−4) for wild-type controls and grin2Ba single mutants, respectively, and of grin2Bb at 3 dpf (n = 4, 4) and 5 dpf (n = 4, 4; *p = 0.037) for wild-type controls and grin2Bb single mutants, respectively. B Relative rt-qPCR expression levels at 3 dpf of grin1a (n = 4, 4; *p = 0.013), grin1b (n = 4, 4; ***p = 9.4e−4), grin2Aa (n = 4, 4), grin2Ab (n = 4, 4; *p = 0.026), grinCa (n = 4, 4), grinCb (n = 4, 4), grinDa (n = 4, 4), and grinDb (n = 4, 4; *p = 0.041) for wild type and grin2B^−/−, respectively. C Relative rt-qPCR expression levels at 3 dpf of grin1a (n = 9, 9; *p = 0.013), grin1b (n = 8, 8; *p = 0.033), grin2Aa (n = 7, 7), grin2Ab (n = 8, 8; **p = 0.0061), grinCa (n = 4, 4), grinCb (n = 4, 4), grinDa (n = 11, 10; **p = 0.0018), and grinDb (n = 11, 11; ***p = 1.5e−4) for wild type and grin2B^−/−, respectively

Expression Data

Genes:	grin1a grin1b grin2ab grin2ba grin2bb grin2da grin2db
Fish:	grin2bb^{sbu311/sbu311} grin2ba^sbu310/+ grin2ba^{sbu310/sbu310}; grin2bb^{sbu311/sbu311}
Anatomical Term:	whole organism
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	grin2ba^{sbu310/sbu310} grin2bb^{sbu311/sbu311} grin2ba^sbu310/+ grin2ba^{sbu310/sbu310}; grin2bb^{sbu311/sbu311}
Observed In:	regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay whole organism
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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