FIGURE

Figure 5

ID

ZDB-FIG-220914-33

Publication

Pietrobono et al., 2022 - p38 MAPK-dependent phosphorylation of transcription factor SOX2 promotes an adaptive response to BRAF inhibitors in melanoma cells

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Figure 5

SOX2 depletion increases sensitivity of melanoma cells to PLX-4032 treatment.A, Western blot of SOX2 in a panel of BRAF^V600E melanoma cells. HSP90 was used as loading control. B and C, dose response curves (B) of PLX-4032 in melanoma cells after 72 h treatment. Table in (C) reports EC50 of PLX-4032 in melanoma cells of at least five independent experiments. D, representative Western blot of SOX2 in A375, SK-MEL-5, and A2058 melanoma cells transduced with LV-c or LV-shSOX2. HSP90 was used as loading control. E and F, dose response curves (E) of A375, SK-MEL-5, and A2058 cells transduced with LV-c or LV-shSOX2 and treated with DMSO or increasing doses of PLX-4032 for 72 h. The EC50 values of PLX-4032 are reported in (F). (N = 3). G, colony formation rate in A375 and A2058 cells transduced with LV-c or LV-shSOX2 and treated with DMSO or increasing doses of PLX-4032 (upper panels). Lower panels show images of colony growth (detected by crystal violet staining). Molecular weight markers are noted next to all immunoblots. p value was calculated by ANOVA and Dunnett’s test (n = 3 biological independent experiments). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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