FIGURE

Figure 3.

ID

ZDB-FIG-220814-3

Publication

Zhang et al., 2022 - A transgenic zebrafish for in vivo visualization of cilia

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Figure 3.

Stable transgenic lines sx1001 effectively marked the ciliary structure in nearly every embryonic tissue. (a) Schematic diagram of transgenic vector construction. (b) Western blot image of Nphp3N-protein expression levels. Protein from wild zebrafish embryos was used as the negative control. (c–f4) The cilia-tagged transgenic zebrafish sx1001 endogenously produced zNphp3N-mCherry to label the ciliary structure in the eyes, otic vesicle, neural tube and trunk. (c–f) Endogenous zNphp3N-mCherry fluorescent fusion proteins (red). (c1–f1) Cilia labelled by anti-AcTub (green). (c2–f2) The basal body of cilia marked by anti-γ-tubulin (grey). (c3–f3) Nucleus stained by Hoechst (blue). (c4–f4) The merge panel. Arrows indicate places with obvious co-localization. The frame denotes the enlarged site. Scale bar, 5 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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