FIGURE

Figure 4

ID

ZDB-FIG-220814-4

Publication

Zhang et al., 2022 - A transgenic zebrafish for in vivo visualization of cilia

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Figure 4

Nphp3N-mCherry integration into sx1001 has no influence on the cilium morphology. (a–d) In all tissues examined, no noticeable difference in cilia morphology was detected between sx1001 and wild-type embryos. (a–d), cilia labelled by anti-AcTub in wild-type embryos at 18 hpf (green). (a′–d′) Cilia marked by sx1001 (red); nuclei were labelled by Hoechst (blue). Scale bar, 5 μm. (e–h) Comparative measurements of the length of cilia in the eyes, otic vesicle, neural tube and myotome from wild-type (black bar) and sx1001 (grey bar) using the maximum intensity projection (MIP) method [33]. Each sample has been measured the length of 20 straight cilia (n = 4 for each sample). Unpaired Student's t-test was used for analysis (n.s., not significant).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sx1001Tg/sx1001Tg (AB)
Observed In:	immature eye cilium myotome cilium neural tube cilium otic vesicle cilium
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

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