Figure 3—figure supplement 3.

(A) Schematic: injection of EnvA-RVΔG-GFP into the OB of adult Tg[gad1b:Gal4;UAS:TVA-mCherry] fish. (B) Example of FACS analysis of GFP and mCherry expression. Boxes depict cells selected as mCherry+/GFP+ (EnvA-RVΔG-GFP infected gad1b neurons), mCherry+/GFP- (non-infected gad1b neurons), mCherry-/GFP- (negative control containing other OB cells). gad1b is one of two isoforms of gad1 that are expressed differentially in GABAergic neurons. (C) Expression of marker genes (x-axis) in infected gad1b neurons (mCherry+/GFP+; green), non-infected gad1b neurons (mCherry+/GFP-; magenta), and other OB cells (mCherry-/GFP-; black). Cells classified as gad1b-positive by fluorescence markers, but not other cells, expressed gad1b but not gad1a, the other gad1 isoform. Expression of fluorescent marker genes followed the detection of fluorescent markers by FACS. The neuronal marker elav3 was present in all three pools. Plot symbols represent data from individual samples; box plots show median and 25^th and 75^th percentiles, circles and error bars indicate mean and s.d. over individual samples (N=8 samples). (D) Expression of negative markers for GABAergic neurons. The selected marker genes (slc17a6a, slc17a6b, tbx21, lhx2b, and lhx9) should be expressed in mitral cells of the OB and other excitatory neurons but not in GABAergic neurons. Consistent with this expectation, expression of all negative markers was low or absent in pools of gad1b cells selected by FACS (N=8 samples).