FIGURE

Figure 1—figure supplement 4.

ID

ZDB-FIG-220726-27

Publication

Satou et al., 2022 - A viral toolbox for conditional and transneuronal gene expression in zebrafish

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Figure 1—figure supplement 4.

HSV1-mediated gene delivery in larvae zebrafish and Gal4/UAS.

(A) Expression of GFP 48 hr after injection of HSV1[LTCMV:GFP] into the optic tectum of zebrafish larvae (3 dpf; maximum intensity projection of confocal stack). Larvae were kept after the injection at 28.5, 32, or 35 °C. (B) Expression of GFP 48 hr after injection of HSV1[LTCMV:GFP] into the optic tectum of a larva at 5 dpf (maximum intensity projection of confocal stack). The larva was kept after the injection at 35 °C. (C) Expression of GFP 48 hr after injection of HSV1[LTCMV:GFP] into the optic tectum of a larva at 14 dpf (maximum intensity projection of confocal stack). The larva was kept after the injection at 35 °C. (D) Expression of GFP 48 hr after injection of HSV1[LTCMV:GFP] into trunk muscles at 7 dpf (maximum intensity projection of confocal stack). The larva was kept after the injection at 35 °C. Note retrograde labeling of motor neurons (M.N.). (E) Expression of GFP 48 hr after injection of HSV1[UAS:GFP] into the hindbrain of a Tg[gad1b:Gal4; gad1b:DsRed] larva at 7 dpf (maximum intensity projection of confocal stack). The larva was kept after the injection at 35 °C. Note co-localization of DsRed and GFP in hindbrain and cerebellum.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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