Figure 3—figure supplement 2.

Temperature-dependence of infection by rabies virus.

(A) Experimental scheme: Rabies virus (EnvA-RVΔG-GFP) was injected into the telencephalon of transgenic fish expressing TVA-mCherry in GABAergic neurons (Tg[gad1b:Gal4; UAS:TVA-mCherry]). (B) Expression of TVA-mCherry and GFP when fish were kept at 26 °C for 6 days after injection. Note almost complete absence of GFP expression.(C) Expression of TVA-mCherry and GFP when fish were kept at 36 °C for 6 days after injection. Note strong GFP expression.(D) Expression of TVA-mCherry and GFP six days after injection when the housing temperature was increased from 26 °C to 36 °C 3 days after injection. GFP expression was weak and sparse.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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