Figure 2—figure supplement 3.

Optogenetic manipulations using HSV1.

(A) Schematic: injection of HSV1[LTCMV:Gal4] into the OB and subsequent co-injection of HSV1[UAS:GCaMP6f] and HSV1[UAS:Chrimson-tdTomato] into the dorsal telencephalon of wildtype fish.(B) Simultaneously recorded calcium transients evoked by optical stimulation of different light intensity (vertical lines) in three example neurons. (C) Mean change GCaMP6f evoked by optical stimulation of different light intensity (11–18 light stimuli at each intensity). Left: GCaMP6f +/tdTomato- neurons (N=8 out of 21 GCaMP6f+ neurons). Right: GCaMP6f +/tdTomato+ neurons (N=13 out 21 GCaMP6f+ neurons). Shading shows SEM. Responses to light stimulation were seen in all individual tdTomato+ neurons (N=13; not shown).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife