PUBLICATION
A viral toolbox for conditional and transneuronal gene expression in zebrafish
- Authors
- Satou, C., Neve, R.L., Oyibo, H.K., Zmarz, P., Huang, K.H., Arn Bouldoires, E., Mori, T., Higashijima, S.I., Keller, G.B., Friedrich, R.W.
- ID
- ZDB-PUB-220723-7
- Date
- 2022
- Source
- eLIFE 11: (Other)
- Registered Authors
- Friedrich, Rainer, Higashijima, Shin-ichi
- Keywords
- gene transfer, imaging, neuronal circuit, neuroscience, viral vector, zebrafish
- MeSH Terms
-
- Animals
- Gene Expression
- Neurons/physiology
- Optogenetics/methods
- Rabies virus*/genetics
- Zebrafish*/genetics
- PubMed
- 35866706 Full text @ Elife
Citation
Satou, C., Neve, R.L., Oyibo, H.K., Zmarz, P., Huang, K.H., Arn Bouldoires, E., Mori, T., Higashijima, S.I., Keller, G.B., Friedrich, R.W. (2022) A viral toolbox for conditional and transneuronal gene expression in zebrafish. eLIFE. 11.
Abstract
The zebrafish is an important model in systems neuroscience but viral tools to dissect the structure and function of neuronal circuitry are not established. We developed methods for efficient gene transfer and retrograde tracing in adult and larval zebrafish by herpes simplex viruses (HSV1). HSV1 was combined with the Gal4/UAS system to target cell types with high spatial, temporal, and molecular specificity. We also established methods for efficient transneuronal tracing by modified rabies viruses in zebrafish. We demonstrate that HSV1 and rabies viruses can be used to visualize and manipulate genetically or anatomically identified neurons within and across different brain areas of adult and larval zebrafish. An expandable library of viruses is provided to express fluorescent proteins, calcium indicators, optogenetic probes, toxins and other molecular tools. This toolbox creates new opportunities to interrogate neuronal circuits in zebrafish through combinations of genetic and viral approaches.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping