Figure 3

Mutation of crlf3 impacts primitive hematopoiesis. (A–AD). Homozygous crlf3^wt/wt (wt/wt), and crlf3^mdu14/mdu14 (mdu14/mdu14) embryos were subjected to WISH at 14 hpf with scl(A, B), fli1(E, F), spi1(I, J) and gata1(M, N), at 20 hpf with ikzf1(P, Q), and at 22 hpf with fli1(S, T), lcp1(V, W), mpo(Y, Z) and hbbe(AB, AC) (scale bar = 200 μm; red and blue dotted areas depict caudal and rostral expression domains, respectively). Individual embryos were assessed for area of staining or cell number at the indicated locations for scl(C, D), fli1(G, H, U), spi1(K, L), gata1(O), ikzf1(R), lcp1(X), mpo(AA) and hbbe(AD), with the mean and SEM shown in red and level of statistical-significance indicated (***p < 0.001, **p < 0.01, *p < 0.05, ns, not significant). Welch’s correction was used for panel (R).