FIGURE

Figure 3

ID

ZDB-FIG-220709-3

Publication

Taznin et al., 2022 - Cytokine Receptor-Like Factor 3 (CRLF3) Contributes to Early Zebrafish Hematopoiesis

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Figure 3

Mutation of crlf3 impacts primitive hematopoiesis. (A–AD). Homozygous crlf3^wt/wt (wt/wt), and crlf3^mdu14/mdu14 (mdu14/mdu14) embryos were subjected to WISH at 14 hpf with scl(A, B), fli1(E, F), spi1(I, J) and gata1(M, N), at 20 hpf with ikzf1(P, Q), and at 22 hpf with fli1(S, T), lcp1(V, W), mpo(Y, Z) and hbbe(AB, AC) (scale bar = 200 μm; red and blue dotted areas depict caudal and rostral expression domains, respectively). Individual embryos were assessed for area of staining or cell number at the indicated locations for scl(C, D), fli1(G, H, U), spi1(K, L), gata1(O), ikzf1(R), lcp1(X), mpo(AA) and hbbe(AD), with the mean and SEM shown in red and level of statistical-significance indicated (***p < 0.001, **p < 0.01, *p < 0.05, ns, not significant). Welch’s correction was used for panel (R).

Expression Data

Genes:	fli1 gata1a ikzf1 lcp1 mpx spi1b tal1
Fish:	WT crlf3^mdu14/mdu14
Anatomical Terms:	endothelial cell hematopoietic multipotent progenitor cell lateral plate mesoderm endothelial cell lateral plate mesoderm erythroid lineage cell lateral plate mesoderm hematopoietic multipotent progenitor cell monocyte myeloid lineage restricted progenitor cell neutrophil
Stage Range:	10-13 somites to 26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	crlf3^mdu14/mdu14
Observed In:	erythroid lineage cell hematopoietic multipotent progenitor cell lateral plate mesoderm erythroid lineage cell monocyte myeloid lineage restricted progenitor cell neutrophil primitive hemopoiesis
Stage Range:	10-13 somites to 26+ somites

Phenotype Detail

Acknowledgments

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