FIGURE

Fig. 7

ID

ZDB-FIG-220627-33

Publication

Della Vecchia et al., 2022 - Trehalose Treatment in Zebrafish Model of Lafora Disease

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Fig. 7

Analysis of autophagy in epm2a^?/? zebrafish mutants. (A) qRT?PCR analysis of autophagy factors (mtor, tfeb, beclin-1, atg5, atg12, lc3, and p62) normalized to ?-actin in epm2a^?/? larvae compared with controls at five dpf. Three independent RNA samples (each obtained from about 30?40 larvae) from controls and epm2a^?/? mutant larvae at 120 hpf were analyzed. * p ? 0.05, ** p ? 0.01, *** p ? 0.001, calculated by Student?s t-test. The values are expressed as mean � standard deviation (SD). (B) Three independent larval homogenates from controls (n = 50) and epm2a^?/? larvae (n = 50) were tested by Western blotting for the expression of ATG5, LC3?I and LC3?II proteins. The levels of the different proteins were normalized to the expression of ?-actin. * p ? 0.05 was calculated by Student?s t-test.

Expression Data

Genes:	atg5 atg12 becn1 map1lc3a mtor sqstm1 tfeb
Antibodies:	Ab2-map1lc3 Ab4-atg5
Fish:	mitfa^w2/+; icm05Tg (AB) mitfa^w2/+; epm2a^{zf3794/zf3794}; icm05Tg (AB)
Anatomical Term:	whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mitfa^w2/+; epm2a^{zf3794/zf3794}; icm05Tg (AB)
Observed In:	whole organism
Stage:	Day 5

Phenotype Detail

Acknowledgments

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