FIGURE

Fig. 2

ID

ZDB-FIG-220627-28

Publication

Della Vecchia et al., 2022 - Trehalose Treatment in Zebrafish Model of Lafora Disease

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Fig. 2

Generation and morphology of epm2a^?/? zebrafish mutants. (A) Coding sequence alignment of wt and mutant epm2a shows the generation of epm2a-null mutant zebrafish by insertion of 5 bp into exon 2 of the epm2a gene. (B) Protein alignment of wt and mutant epm2a. The red rectangle shows the premature stop codon at residue 74 (p.Thr54Asnfs*74) caused by the 5-bp insertion mutation in exon 2. (C) qRT?PCR analysis revealed a decrease in the level of epm2a mRNA expression, normalized to the expression of ?-actin mRNA. Three independent RNA samples (each obtained from about 30?40 larvae) from epm2a^?/? larvae at 120 hpf and from controls were analyzed. The values are expressed as mean � standard deviation (SD). **, p ? 0.01, calculated by Student?s t-test. (D) Lateral view photographs of representative control and epm2a^?/? specimens. At 4 dpf we observed a significant reduction in body length in epm2a^?/? larvae (n = 83) compared to WT controls (n = 83), **** p ? 0.0001, calculated by Mann-Whitney test.

Expression Data

Gene:	epm2a
Fish:	mitfa^w2/+; icm05Tg (AB) mitfa^w2/+; epm2a^{zf3794/zf3794}; icm05Tg (AB)
Anatomical Term:	whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mitfa^w2/+; epm2a^{zf3794/zf3794}; icm05Tg (AB)
Observed In:	whole organism
Stage Range:	Day 4 to Day 5

Phenotype Detail

Acknowledgments

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