FIGURE

FIGURE 1

ID

ZDB-FIG-220612-1

Publication

Zhang et al., 2022 - Connectivity Mapping Using a Novel sv2a Loss-of-Function Zebrafish Epilepsy Model as a Powerful Strategy for Anti-epileptic Drug Discovery

Other Figures

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FIGURE 1

sv2a expression in developing zebrafish larvae (1-7 dpf) and CRISPR-mediated sv2a knockout in zebrafish. (A) qPCR analysis of sv2a mRNA levels in wildtype larvae normalized to 18s and represented as the fold expression to 1 dpf. Values are reported as mean ± SD of three independent experiments. (B) Schematic representation of the SV2A protein with the two distinct transmembrane (TM) domains and the cytosolic synaptogamin binding site. Gray dashed line indicates exon 1 encoding synaptogamin binding domain and the first two TM helices. Red dashed line magnifies the region with the 1 nucleotide insertion (G) at the target site in exon 1. (C) High resolution melting (HRM) curve analysis discriminates sv2a^+/+, sv2a^+/–, and sv2a^–/– zebrafish larvae at 6 dpf. (D) qPCR analysis of sv2a mRNA levels in sv2a^+/+, sv2a^+/–, and sv2a^–/– larvae at 6 dpf normalized to 18s and represented as the fold expression to sv2a^+/+ larvae. Values are reported as the mean ± SD of three independent experiments.

Expression Data

Gene:	sv2a
Fish:	AB sv2a^lv105/lv105 (AB) sv2a^lv105/+ (AB)
Anatomical Term:	whole organism
Stage Range:	Prim-5 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sv2a^lv105/lv105 (AB) sv2a^lv105/+ (AB)
Observed In:	whole organism
Stage:	Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.