IMAGE

FIGURE 1

ID

ZDB-IMAGE-220612-1

Genes

sv2a

Publication

Zhang et al., 2022 - Connectivity Mapping Using a Novel sv2a Loss-of-Function Zebrafish Epilepsy Model as a Powerful Strategy for Anti-epileptic Drug Discovery

All Figures

Figures for Zhang et al., 2022

Image

Figure Caption

FIGURE 1

sv2a expression in developing zebrafish larvae (1-7 dpf) and CRISPR-mediated sv2a knockout in zebrafish. (A) qPCR analysis of sv2a mRNA levels in wildtype larvae normalized to 18s and represented as the fold expression to 1 dpf. Values are reported as mean ± SD of three independent experiments. (B) Schematic representation of the SV2A protein with the two distinct transmembrane (TM) domains and the cytosolic synaptogamin binding site. Gray dashed line indicates exon 1 encoding synaptogamin binding domain and the first two TM helices. Red dashed line magnifies the region with the 1 nucleotide insertion (G) at the target site in exon 1. (C) High resolution melting (HRM) curve analysis discriminates sv2a^+/+, sv2a^+/–, and sv2a^–/– zebrafish larvae at 6 dpf. (D) qPCR analysis of sv2a mRNA levels in sv2a^+/+, sv2a^+/–, and sv2a^–/– larvae at 6 dpf normalized to 18s and represented as the fold expression to sv2a^+/+ larvae. Values are reported as the mean ± SD of three independent experiments.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.