FIGURE

Figure 5

ID

ZDB-FIG-220416-55

Publication

Huang et al., 2022 - Negative Elongation Factor (NELF) Inhibits Premature Granulocytic Development in Zebrafish

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Figure 5

Other NELF subunits play similar roles in primitive granulocytic development. (A) Q-RT-PCR analysis of nelfa and nelfe expression at different stages. The relative gene expression was normalized to β-actin. (B) WISH for scl, pu.1, mpx, mfap4, or gata1 in WT, nelfa, or nelfe morphants at 22 hpf. Yellow arrowheads and red arrowheads, respectively, indicate ALPM and PLPM. (C–G) Quantification of WISH results in (B) (n = 30–50 embryos per group). All results are presented as the mean ± SD from three independent experiments t test, * for p < 0.05, ** for p < 0.01, *** for p < 0.001, **** for p < 0.0001). “+++” and “+” in (C–G) respectively represent strong staining and weak staining.

Expression Data

Genes:	gata1a mfap4.1 mpx nelfa nelfe spi1b tal1
Fish:	AB AB + MO1-nelfa AB + MO1-nelfe
Knockdown Reagents:	MO1-nelfa MO1-nelfe
Anatomical Terms:	common myeloid progenitor erythroid lineage cell hematopoietic multipotent progenitor cell macrophage neutrophil whole organism
Stage Range:	5-9 somites to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-nelfa AB + MO1-nelfe
Knockdown Reagents:	MO1-nelfa MO1-nelfe
Observed In:	macrophage neutrophil
Stage:	26+ somites

Phenotype Detail

Acknowledgments

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