FIGURE

Figure 4

ID

ZDB-FIG-220416-22

Publication

Huang et al., 2022 - Negative Elongation Factor (NELF) Inhibits Premature Granulocytic Development in Zebrafish

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Figure 4

Expression of human NELFB in hematopoietic cells partially rescues granulopoiesis in nelfb^−/− embryos. (A) Alignment of human, mouse, and zebrafish NELFB proteins. Identical and similar amino acids are shaded in gray. (B) Generation of Tg(drl:hNELFB-2A-GFP) fish and GFP expression. (C) WISH for scl or pu.1 in WT and Tg(drl:hNELFB-2A-GFP) embryos at 22 hpf. (D) Quantification of WISH results in (C) (n = 40–60 embryos per group). (E) WISH for mpx in WT, Tg(drl:hNELFB-2A-GFP), nelfb^−/− and nelfb^−/−; Tg(drl:hNELFB-2A-GFP) embryos at 22 hpf. (F) Quantification of WISH results in (E) (n = 20–30 embryos per group). All results are presented as the mean ± SD from three independent experiments (t test, * for p < 0.05). “+++” and “+” in (D,F) respectively represent strong staining and weak staining. hNELFB, human NELFB.

Expression Data

Genes:	mpx spi1b tal1
Fish:	AB nelfb^{zf3621/zf3621} (AB) zf3623Tg (AB) nelfb^{zf3621/zf3621}; zf3623Tg (AB)
Anatomical Terms:	common myeloid progenitor hematopoietic multipotent progenitor cell neutrophil
Stage:	26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nelfb^{zf3621/zf3621} (AB) zf3623Tg (AB) nelfb^{zf3621/zf3621}; zf3623Tg (AB)
Observed In:	granulocyte granulocyte differentiation neutrophil
Stage:	26+ somites

Phenotype Detail

Acknowledgments

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